منابع مشابه
A simplified preparation of phosphatidyl inositol.
A method is described for the rapid isolation of phosphatidyl inositol from soybean phosphatides (Asolectin). The product is obtained pure as the crystalline sodium salt.
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The unidirectional digestion of DNA, first cut with two different restriction enzymes generating one 3'-protrusion and than with exonuclease III and Sl-nuclease allows a very defined removal of DNA for sequencing (1,2) or mapping of biological functions. The DNA is cloned into the polylinker of e.g. pUC18 or mpl8 (2), double digested with SstI/SmaI or PstI/XbaI, phenol extracted, ethanol precip...
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Serving as a DNA molecular weight standard, the DNA ladder has been widely used in molecular biology applications. We developed a simple method for the preparation of a DNA marker, which involves designing primers to amplify 100- to 1000-bp DNA fragments using lambda DNA as a template for polymerase chain reaction, followed by extraction with phenol/chloroform, precipitation with ethanol and mi...
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ژورنال
عنوان ژورنال: SSRN Electronic Journal
سال: 2011
ISSN: 1556-5068
DOI: 10.2139/ssrn.1882047